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<t>Fkbp5+</t> cells in the ovBNST coexpress with the neuropeptides Crh and Tac2 and their number is significantly increased after exposure to ASR. A , Fkbp5 and Tac2 are coexpressed in the ovBNST, as can be seen in detail (violet outline arrow). Fkbp5 and Crh are also coexpressed in the ovBNST as shown in detail (violet arrow). B , Expression patterns of Fkbp5 with Tac2 and Crh in the ovBNST also strongly overlapped (gray outline arrow). In addition, there were some cells that expressed Fkbp5 only (gray arrow). C , Quantification of the number of cells expressing Fkbp5 only, coexpressing Fkbp5 and Tac2 , coexpressing Fkbp5 and Crh , and coexpressing Fkbp5 , Tac2 , and Crh after exposure to ASR resulted in significant upregulation across all cell types. Scale bar ( A , B ): 25 μm. Data are mean ± SEM; * p < 0.05, ** p < 0.01.
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aav1 chemicals  (Addgene inc)
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<t>Fkbp5+</t> cells in the ovBNST coexpress with the neuropeptides Crh and Tac2 and their number is significantly increased after exposure to ASR. A , Fkbp5 and Tac2 are coexpressed in the ovBNST, as can be seen in detail (violet outline arrow). Fkbp5 and Crh are also coexpressed in the ovBNST as shown in detail (violet arrow). B , Expression patterns of Fkbp5 with Tac2 and Crh in the ovBNST also strongly overlapped (gray outline arrow). In addition, there were some cells that expressed Fkbp5 only (gray arrow). C , Quantification of the number of cells expressing Fkbp5 only, coexpressing Fkbp5 and Tac2 , coexpressing Fkbp5 and Crh , and coexpressing Fkbp5 , Tac2 , and Crh after exposure to ASR resulted in significant upregulation across all cell types. Scale bar ( A , B ): 25 μm. Data are mean ± SEM; * p < 0.05, ** p < 0.01.
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viruses  (Addgene inc)
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<t>Fkbp5+</t> cells in the ovBNST coexpress with the neuropeptides Crh and Tac2 and their number is significantly increased after exposure to ASR. A , Fkbp5 and Tac2 are coexpressed in the ovBNST, as can be seen in detail (violet outline arrow). Fkbp5 and Crh are also coexpressed in the ovBNST as shown in detail (violet arrow). B , Expression patterns of Fkbp5 with Tac2 and Crh in the ovBNST also strongly overlapped (gray outline arrow). In addition, there were some cells that expressed Fkbp5 only (gray arrow). C , Quantification of the number of cells expressing Fkbp5 only, coexpressing Fkbp5 and Tac2 , coexpressing Fkbp5 and Crh , and coexpressing Fkbp5 , Tac2 , and Crh after exposure to ASR resulted in significant upregulation across all cell types. Scale bar ( A , B ): 25 μm. Data are mean ± SEM; * p < 0.05, ** p < 0.01.
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<t>Fkbp5+</t> cells in the ovBNST coexpress with the neuropeptides Crh and Tac2 and their number is significantly increased after exposure to ASR. A , Fkbp5 and Tac2 are coexpressed in the ovBNST, as can be seen in detail (violet outline arrow). Fkbp5 and Crh are also coexpressed in the ovBNST as shown in detail (violet arrow). B , Expression patterns of Fkbp5 with Tac2 and Crh in the ovBNST also strongly overlapped (gray outline arrow). In addition, there were some cells that expressed Fkbp5 only (gray arrow). C , Quantification of the number of cells expressing Fkbp5 only, coexpressing Fkbp5 and Tac2 , coexpressing Fkbp5 and Crh , and coexpressing Fkbp5 , Tac2 , and Crh after exposure to ASR resulted in significant upregulation across all cell types. Scale bar ( A , B ): 25 μm. Data are mean ± SEM; * p < 0.05, ** p < 0.01.
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Image Search Results


Fkbp5+ cells in the ovBNST coexpress with the neuropeptides Crh and Tac2 and their number is significantly increased after exposure to ASR. A , Fkbp5 and Tac2 are coexpressed in the ovBNST, as can be seen in detail (violet outline arrow). Fkbp5 and Crh are also coexpressed in the ovBNST as shown in detail (violet arrow). B , Expression patterns of Fkbp5 with Tac2 and Crh in the ovBNST also strongly overlapped (gray outline arrow). In addition, there were some cells that expressed Fkbp5 only (gray arrow). C , Quantification of the number of cells expressing Fkbp5 only, coexpressing Fkbp5 and Tac2 , coexpressing Fkbp5 and Crh , and coexpressing Fkbp5 , Tac2 , and Crh after exposure to ASR resulted in significant upregulation across all cell types. Scale bar ( A , B ): 25 μm. Data are mean ± SEM; * p < 0.05, ** p < 0.01.

Journal: eNeuro

Article Title: FKBP51 in the Oval Bed Nucleus of the Stria Terminalis Regulates Anxiety-Like Behavior

doi: 10.1523/ENEURO.0425-21.2021

Figure Lengend Snippet: Fkbp5+ cells in the ovBNST coexpress with the neuropeptides Crh and Tac2 and their number is significantly increased after exposure to ASR. A , Fkbp5 and Tac2 are coexpressed in the ovBNST, as can be seen in detail (violet outline arrow). Fkbp5 and Crh are also coexpressed in the ovBNST as shown in detail (violet arrow). B , Expression patterns of Fkbp5 with Tac2 and Crh in the ovBNST also strongly overlapped (gray outline arrow). In addition, there were some cells that expressed Fkbp5 only (gray arrow). C , Quantification of the number of cells expressing Fkbp5 only, coexpressing Fkbp5 and Tac2 , coexpressing Fkbp5 and Crh , and coexpressing Fkbp5 , Tac2 , and Crh after exposure to ASR resulted in significant upregulation across all cell types. Scale bar ( A , B ): 25 μm. Data are mean ± SEM; * p < 0.05, ** p < 0.01.

Article Snippet: Manipulation of FKBP51 was performed using adeno-associated bicistronic AAV1/2 vectors (AAV1/2-HA-CAG-FKBP5-1 and AAV1/2-CAG-null, GeneDetect; AAV1-CMV-Cre and AAV1/2-CAG-empty-IRES-EGFP, Addgene; AAV1/2-ESARE-ER T2 CreER T2 ).

Techniques: Expressing